摘要:目的:研究茯苓的不同產(chǎn)地茯苓質(zhì)量,為茯苓質(zhì)量標準的建立及其產(chǎn)地開發(fā)利用提供依據(jù)。方法:(1)按照《中國藥典》2015版相關(guān)方法測定茯苓中水分、灰分、浸出物含量。(2)利用紫外-可見分光光度法測定茯苓中的水溶性多糖及茯苓總?cè)频暮?。?)利用高效液相色譜法測定茯苓中的茯苓酸的含量。結(jié)果:貴州統(tǒng)塊的浸出物、茯苓總?cè)?、茯苓酸含量最高。云南的茯苓浸出物?.39~3.99%之間,茯苓總?cè)坪吭?.25~0.30%、茯苓酸含量0.07~0.09%之間,茯苓多糖含量在0.21~0.42之間。除茯苓多糖含量外整體相差不大。安徽的茯苓浸出物在2.65~4.01%之間,茯苓總?cè)坪吭?.17~0.31%、茯苓酸含量0.05~ 0.08%之間,茯苓多糖含量0.13~0.42之間。
關(guān)鍵詞:茯苓,質(zhì)量,茯苓多糖,茯苓總?cè)?br />
Abstract: Objective: To study the quality of Poria from different areas, and to provide basis for the establishment of quality standards of Poria and its development and utilization. Methods: (1) The contents of water, ash and extractives in Poria cocos are determined according to the relevant methods of Chinese Pharmacopoeia 2015. (2) UV-visible spectrophotometry was used to determine the content of water-soluble polysaccharides and total triterpenes in Poria cocos. (3) The content of Poria acid in Poria was determined by high performance liquid chromatography. Results: The extracts, total triterpenoids,and Poria acid from Guizhou block were the highest. The extract of Poria from Yunnan is between 3.39% and 3.99%, the total triterpene content of Poria is from 0.25% to 0.30%, and the content of Poria acid is from0.07 to 0.09%. The polysaccharide content of Poria is between 0.21 and 0.42. Except for the polysaccharide content of Poria, the overall difference was not significant. The extracts of Poria from Anhui ranged from 2.65% to 4.01%, the total triterpenes content of Poria from 0.17% to 0.31%, the content of Poria acid from 0.05% to 0.08%, and Poria’s polysaccharide content is between 0.13 and 0.42. The content of Poria from Anhui Province is quite different. The extract of Poria from Hubei is between 3.03% and 3.44%, the total triterpene content of Poria is between 0.22% and 0.25%, the content of Poria is 0.05% to 0.07%, and Poria’s polysaccharide content is between 0.11 and 0.22. The content of Poria in Hubei is not much different.
Keywords: the quality of Poria, polysaccharide, Poria total triterpenes