黃達(dá)明; 李海龍; 張志才; 崔鳳杰
江蘇大學(xué)食品與生物工程學(xué)院

【中文摘要】 通過優(yōu)化培養(yǎng)基組成,提高雞腿菇發(fā)酵液對非酶糖基化反應(yīng)的抑制活性。試驗以抑制非酶糖基化反應(yīng)活性為指標(biāo),通過單因素試驗篩選了適宜碳源、氮源、無機(jī)鹽以及復(fù)合營養(yǎng)因子,通過正交試驗進(jìn)一步優(yōu)化培養(yǎng)基組成。試驗結(jié)果表明,使用優(yōu)化培養(yǎng)基[麥芽糖4%,牛肉膏0.6%,黃豆芽(煮汁)200 g/L,洋蔥(煮汁)100 g/L,MgSO40.12%,FeSO40.10%,CuSO40.03%,ZnSO40.05%]液體培養(yǎng)雞腿菇,其發(fā)酵液對非酶糖基化反應(yīng)的抑制率高達(dá)97.32%。

【英文摘要】 A culture medium for Coprinus comatus has been designed whereby the non-enzymatic glycation-inhibiting activity of the fermentation broth was maximized.Different carbon and nitrogen sources,inorganic salts and natural components were screened initially using single factor experiments,followed by orthogonal tests designed on the basis of the data obtained.The highest inhibition rate(97.32%) was achieved using a culture medium composed of maltose 4%,beef extract 0.6%,soybean sprout juice 200 g/L,onion juice 100 g/L,magnesium sulfate 0.12%,ferrous sulfate 0.1%,copper sulfate 0.03% and zinc sulfate 0.05%.

【中文關(guān)鍵詞】 雞腿菇; 抑制非酶糖基化反應(yīng)活性; 培養(yǎng)基優(yōu)化
【英文關(guān)鍵詞】 Coprinus comatus; non-enzymatic glycation-inhibiting activity; optimization of culture medium
【基金】南通安惠生物工程有限公司委托課題“藥用真菌轉(zhuǎn)化富集桑葉活性成分”的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-013