為了充分開(kāi)發(fā)利用靈芝資源，本研究對(duì)采自西藏墨脫縣的一份野生靈芝子實(shí)體標(biāo)本進(jìn)行了分離純化培養(yǎng)，并通過(guò)形態(tài)學(xué)觀察和分子生物學(xué)分析鑒定為有柄靈芝Ganoderma gibbosum。將此菌株作為試驗(yàn)菌株，從碳源、氮源、溫度、pH這4個(gè)因素進(jìn)行生物學(xué)特性研究，從中選擇3個(gè)較優(yōu)水平進(jìn)行正交試驗(yàn)。結(jié)果表明，有柄靈芝菌絲生長(zhǎng)的最佳碳源為果糖，氮源為酵母，最適溫度為25 ℃，適宜pH為7.0。馴化出芝栽培基質(zhì)配方選用雜木屑58%、玉米芯20%、麥麩20%、石灰1%、石膏1%，40 d左右菌絲滿(mǎn)袋，覆土一周后出現(xiàn)原基，50 d左右子實(shí)體成熟。初步成分分析表明，有柄靈芝粗多糖、氨基酸含量分別為1.5%和10.8%。本試驗(yàn)成功對(duì)野生有柄靈芝進(jìn)行了人工馴化栽培，為有柄靈芝作為新的藥用菌進(jìn)行開(kāi)發(fā)利用提供了參考。

A wild strain of Ganoderma from Medog, Xizang, was identified as Ganoderma gibbosum, based on morphological observation and molecular biological analysis. Its biological characteristics and domestic cultivation were studied. Single-factor tests were conducted for optimizing carbon and nitrogen sources, temperature and pH under solid cultivation conditions. Orthogonal experiment of three optimal levels selected from the four single-factor tests showed that the optimal carbon source and nitrogen source for mycelial growth of G. gibbosum were fructose and yeast, respectively. The optimum temperature was 25 ℃, and the suitable pH was 7.0. The fruiting bodies of G. gibbosum could be formed on the substrate formulated by 58% angiosperm wood chips, 20% corncob, 20% bran, 1% lime, and 1% gypsum. The hyphae fully colonized the substrate after cultivation for 40 days. The primordium began to form in one week after casing, and the fruiting body matured in about 50 days. Preliminary analysis of functional components indicated that polysaccharide and amino acid contents of G. gibbosum were 1.5% and 10.8%, respectively. G. gibbosum might have a new potential medicinal value in future applications.